Molecular basis of the autoregulatory mechanism of motor neuron-related splicing factor 30.

ABSTRACT: Motor neuron-related splicing factor 30 (SPF30, also known as SMNDC1) is a paralog of the survival motor neuron protein that regulates the expression of various genes by affecting mRNA splicing. SPF30 has an autoregulatory mechanism that controls its expression. However, the detailed molecular mechanisms determining cellular levels of SPF30 remain unclear. Here, we demonstrated that SPF30 expression was controlled via the negative autoregulatory feedback, whereby increased SPF30 expression caused the inclusion of cassette exon within intron 2 and/or the generation of a newly spliced variant with exon 4a (produced by splicing 17 bases upstream of the canonical intron 3 and exon 4 junction). Altered transcripts with cassette exon or exon 4a were subjected to nonsense-mediated mRNA decay, lea

SUBMITTER: Izumikawa K 

PROVIDER: S-EPMC12398788 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature