Effects of 1, 25-dihydroxy-vitamin D₃ on the immune response in chicken embryo fibroblast cells infected by infectious bronchitis virus.

ABSTRACT: In addition to its nutritional role in regulating calcium and phosphorus metabolism, vitamin D also possesses immunoregulatory effects. Herein, we used chicken embryo fibroblast cells (CEFs) to study the effect of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) on the immune response induced by infectious bronchitis virus (IBV) infection. We observed the inhibition to virus proliferation in the cells by adding 10 nM 1,25(OH)2D3. In addition, treatment with 1,25(OH)2D3 has significantly increased the mRNA expressions of toll-like receptor 15 (TLR15) (2.75-fold), avian beta-defensins 9 (AvBD9) (25.37-fold), and AvBD14 (2.28-fold) in the IBV-infected CEFs comparing with those of the control group. In contrast, the mRNA expression levels of TLR3 (0.98-fold), TLR4 (0.66-fold), TLR5 (0.44-fold), TLR7 (0.

SUBMITTER: Wang F 

PROVIDER: S-EPMC12398814 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature