Three-dimensional cell sheet model improves <i>in vitro</i> prediction accuracy of osteogenic potential for biodegradable magnesium-based metals.

ABSTRACT: Biodegradable metals have been increasingly utilized clinically due to their biosafety and pro-osteogenic properties. However, conventional monolayer cell-based preclinical safety evaluation methods based on ISO10993-5 consistently indicate significant cytotoxicity that contradicts in vivo outcomes. In this study, we aimed to establish an in vitro evaluation model that better correlates with in vivo performance. Three-layer BMSC cell sheets were constructed using layer-by-layer assembly. Histological analyses revealed a stable three-dimensional structure with elevated cell-cell interaction proteins, including N-Cadherin, Fibronectin, and Vinculin, along with enhanced osteogenic potential. The cytotoxicity of 4N pure Mg was evaluated in both cell sheet and monolayer co-

SUBMITTER: Chen L 

PROVIDER: S-EPMC12398864 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature