Role of ebv-circRPMS1-p53 interaction in the proliferation and clinical progression of Epstein-Barr virus-associated gastric carcinoma.

ABSTRACT: Epstein-Barr virus-associated gastric carcinoma (EBVaGC) represents a distinct clinicopathological entity with unique molecular characteristics, including latent EBV infection and dysregulation of host tumor suppressors. However, the functional interplay between EBV circular RNAs (ebv-circRNAs) and p53 protein remains enigmatic. This study explored the role of ebv-circRPMS1, a previously uncharacterized ebv-circRNA, in EBVaGC pathogenesis. To define ebv-circRPMS1-p53 interplay, siRNA knockdown (validated by RT-qPCR) modulated ebv-circRPMS1 in EBV+ cells. RIP/co-immunoprecipitation confirmed direct ebv-circRPMS1-p53 binding. EdU assays quantified proliferation. RNA-FISH/immunofluorescence mapped cytoplasmic colocalization. Xenografts evaluated in vivo tumorigenicity, while BaseScope/IHC ana

SUBMITTER: Yang L 

PROVIDER: S-EPMC12398897 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature