IMDM-20 enhances neutrophilic features during 1.3 % DMSO-mediated differentiation of HL-60 cells.

ABSTRACT: The promyelocytic HL-60 cell line can be differentiated toward neutrophil-like cells and has been historically used as a surrogate to study human neutrophil biology in vitro. Multiple differentiation protocols have been reported to generate neutrophil-like HL-60 cells, with limited consideration of how methodological variations might influence cell identity and functions. Here, we conducted a systematic search of the PubMed database, to investigate the current heterogeneity in published protocols used to differentiate HL-60 towards neutrophil-like cells. Research articles published in English between January 9th^, 2020, and January 9th^, 2025, were identified using the key words "neutrophil-like cell" and "HL-60". Metadata of included research papers were charted

SUBMITTER: Cazares-Preciado JA 

PROVIDER: S-EPMC12398911 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature