Overexpression of miRNA-216 in exosomes derived from umbilical cord mesenchymal stem cells promotes angiogenesis and improves functional recovery after spinal cord injury.

ABSTRACT:

Objectives

This study aimed to engineer miR-216-overexpressing umbilical cord mesenchymal stem cells (UCMSCs) to generate miR-216-enriched UCMSC-derived exosomes (UCMSC-Exos) and evaluate their therapeutic potential in Spinal cord injury (SCI).

Materials and methods

miR-216 overexpression was achieved in UCMSCs, and exosomes were subsequently isolated. The biological effects of miR-216-overexpressing UCMSC-Exos (UCMSC-miR-216^OE-Exos) were assessed using in vitro migration, and tube formation assays with vascular endothelial cells. For in vivo evaluation, SCI mouse models were treated with either UCMSC-Exos or UCMSC-miR-216^OE-Exos. Functional recovery was measured using the BMS scores, while angiogenesis, neuronal apoptosis, and proinflamma

SUBMITTER: Li H 

PROVIDER: S-EPMC12399067 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature