MultNAT HIV-1 Viral Load Assay: a simple, rapid and sensitive cross-primer amplification assay for detecting the HIV-1 virus in the field.

ABSTRACT:

Background

A large number of new human immunodeficiency virus (HIV) patients are diagnosed worldwide every year. This study aimed to design a rapid, efficient, and convenient kit for detecting HIV-1 viral load.

Methods

The sensitivity and specificity of the kit were determined, and serum samples were collected from HIV-negative and HIV-positive individuals who underwent medical checkups at Xixi Hospital in Hangzhou City, Zhejiang Province, China, between June and August 2023 for testing. The presence of HIV-1 in the clinical samples was assessed via a diagnostic PCR system and a conventional fluorescent quantitative PCR technique in this study.

Results

HIV-1 RNA was measured with a 100 copies/mL sensitivity via the MultNAT HIV-1 Viral Load Assay. The acc

SUBMITTER: Huang L 

PROVIDER: S-EPMC12399072 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature