Fish and chips: various methodologies demonstrate utility of a 16,006-gene salmonid microarray.

ABSTRACT:

Background

We have developed and fabricated a salmonid microarray containing cDNAs representing 16,006 genes. The genes spotted on the array have been stringently selected from Atlantic salmon and rainbow trout expressed sequence tag (EST) databases. The EST databases presently contain over 300,000 sequences from over 175 salmonid cDNA libraries derived from a wide variety of tissues and different developmental stages. In order to evaluate the utility of the microarray, a number of hybridization techniques and screening methods have been developed and tested.

Results

We have analyzed and evaluated the utility of a microarray containing 16,006 (16K) salmonid cDNAs in a variety of potential experimental settings. We quantified the amount of transcriptome binding that occurred

SUBMITTER: von Schalburg KR 

PROVIDER: S-EPMC1239916 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature