Epigenetic inactivation and aberrant transcription of CSMD1 in squamous cell carcinoma cell lines.

ABSTRACT:

Background

The p23.2 region of human chromosome 8 is frequently deleted in several types of epithelial cancer and those deletions appear to be associated with poor prognosis. Cub and Sushi Multiple Domains 1 (CSMD1) was positionally cloned as a candidate for the 8p23 suppressor but point mutations in this gene are rare relative to the frequency of allelic loss. In an effort to identify alternative mechanisms of inactivation, we have characterized CSMD1 expression and epigenetic modifications in head and neck squamous cell carcinoma cell lines.

Results

Only one of the 20 cell lines examined appears to express a structurally normal CSMD1 transcript. The rest express transcripts which either lack internal exons, terminate abnormally or initiate at cryptic promoters. None of the

SUBMITTER: Richter TM 

PROVIDER: S-EPMC1239921 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature