From gut to proteomics: the impact of <i>Roseburia intestinalis</i> on post-translational modifications in colorectal cancer.

ABSTRACT:

Background

Colorectal cancer (CRC) represents a significant global health challenge. Gut microbiota imbalance and abnormal chromatin modifications play critical roles in the progression of CRC. However, the mechanisms by which they exert their influences, particularly the involvement of Roseburia intestinalis (R.i)-mediated post-translational modifications (PTMs), remain inadequately understood. This study aims to explore global acylase change map induced by R.i in CRC cells.

Methods

The 4D-Fast DIA quantitative acetylated modified proteome and the 4D-Fast DIA quantitative lactated modified proteome combined with proteomics were used to detect CRC cells (HCT116) co-cultured with R.i. Cell proliferation was evaluated by the Cell Counting Kit-8 (CCK

SUBMITTER: Li L 

PROVIDER: S-EPMC12399380 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature