Aquaporin 2 is differentially expressed in granulosa cells of various stages of human follicles and is regulated by luteinizing hormone.

ABSTRACT:

Introduction

Several aquaporins (AQPs) are involved in the influx of water to form follicular fluid, and AQP2 may play a crucial role in follicular growth. However, the specific roles of Aquaporin (AQP) 2 and AQP6 in granulosa cells (GCs) during follicular fluid (FF) formation, as well as their relationship with gonadotropins (Gn), remain unclear.

Methods

Luteinized granulosa cells (LGCs) were isolated from follicles of different diameters. Western blot indicated that AQP2 protein levels in LGCs increased as follicles grew larger after luteinization. Immunohistochemistry of human ovarian sections showed that AQP2 levels decreased as follicles progressed from primordial to antral stages. Subsequently, isolated LGCs were treated with varying concentrations of follicle-stimulat

SUBMITTER: Zheng Z 

PROVIDER: S-EPMC12399404 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature