EVOPRINTER, a multigenomic comparative tool for rapid identification of functionally important DNA.

ABSTRACT: Here, we describe a multigenomic DNA sequence-analysis tool, evoprinter, that facilitates the rapid identification of evolutionary conserved sequences within the context of a single species. The evoprinter output identifies multispecies-conserved DNA sequences as they exist in a reference DNA. This identification is accomplished by superimposing multiple reference DNA vs. test-genome pairwise blat (blast-like alignment tool) readouts of the reference DNA to identify conserved nucleotides that are shared by all orthologous DNAs. evoprinter analysis of well characterized genes reveals that most, if not all, of the conserved sequences are essential for gene function. For example, analysis of orthologous genes that are shared by many vertebrates identifies conserved DNA in both protein-encodin

SUBMITTER: Odenwald WF 

PROVIDER: S-EPMC1239946 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature