Exosomal miR-93-3p targets EIF4EBP1 to regulate macrophage polarization and accelerate wound healing post-anal fistula surgery.

ABSTRACT:

Background

Delayed wound healing following anal fistula (AF) surgery remains a clinical challenge. This study endeavors to identify and validate key exosomal miRNAs that regulate postoperative inflammation after AF surgery by integrating multi-omics analyses with functional assays, and to elucidate the molecular mechanisms by which these miRNAs and their target genes influence macrophage M1/M2 polarization.

Methods

15 patients undergoing AF surgery were randomized to three groups. The negative control group received sterile Vaseline gauze dressings, the positive control cohort took Kangfuxin Solution, and the treatment cohort received Wugu Qilin Ointment. Wound exudates were collected postoperatively and exosomes were isolated via ultracentrifugation. Total RNA was extracted

SUBMITTER: Zhang W 

PROVIDER: S-EPMC12399553 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature