Evaluation of targeted next-generation sequencing for microbiological diagnosis of acute lower respiratory infection.

ABSTRACT:

Purpose

To evaluate the performance of targeted next-generation sequencing (tNGS) in pathogen detection in acute lower respiratory infection.

Methods

The retrospective study was conducted between July 2023 and May 2024 at the Yantai Yuhuangding Hospital. Patients with acute lower respiratory infections were included. Qualified sputum or bronchoalveolar lavage fluid samples were collected for tNGS and conventional microbiological tests(CMTs), including culture, staining, polymerase chain reaction (PCR), and reverse transcription-PCR (RT-PCR). The time required and cost were counted.

Results

A total of 968 patients were enrolled. Study analysis discovered 1,019 strains of bacteria, 259 strains of fungi, 302 strains of viruses, 76 strains of Mycoplasma pneumoniae,

SUBMITTER: Yang F 

PROVIDER: S-EPMC12399591 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature