Immune counter-evolution: immortalized B cell clones can undergo ex vivo directed evolution to counteract viral escape.

ABSTRACT:

Introduction

Amid the persistent threat of future pandemics, the continuous evolution of SARS-CoV-2 exposed critical challenges for vaccine efficacy and therapeutic interventions, highlighting the need for rapid and adaptable approaches to respond to immune escape variants.

Methods

Here, we report the use of immortalized B cell libraries from human peripheral blood mononuclear cells (PBMCs) and tonsil tissues to uncover B cell clones exhibiting cross-reactive neutralization against various SARS-CoV-2 variants and perform directed evolution of immortalized B cell clones to produce antibodies with improved binding and neutralization against emerging SARS-CoV-2 variants.

Results

Immortalization of PBMC and tonsil-derived human B cells was achieved through transduction wi

SUBMITTER: Marsman C 

PROVIDER: S-EPMC12399645 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature