Identification and Characterization of Glycine- and Proline-Rich Antioxidant Peptides From Antler Residues Based on Peptidomics, Machine Learning, and Molecular Docking.

ABSTRACT: The purpose of this study was to determine the optimal enzymatic hydrolysis process for antler residues and to identify and characterize the antioxidant peptides present in the products of this hydrolysis. The results indicated that when neutrase is employed as the hydrolase, with an enzyme concentration of 2300 U/g, a temperature of 45°C, a pH of 6.0, a hydrolysis duration of 7.5 h, and a water/material ratio of 15%, the antler residual peptide (ARP) exhibited the highest DPPH clearance rate and degree of hydrolysis. Characterization of the physical and chemical properties revealed that ARP is a mixed antioxidant peptide rich in glycine and proline, with 93.6% of its composition consisting of peptides with a molecular weight of lower than 1 kDa. Furthermore, 21 antioxidant peptides were s

SUBMITTER: Xiao X 

PROVIDER: S-EPMC12400163 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature