Peptide nucleic acids in parallel orientation form invasion complexes with double-stranded DNA.

ABSTRACT: Peptide nucleic acid (PNA) is a unique class of synthetic nucleic acids with a pseudo-peptide backbone, known for its high nucleic acid recognition capability and its ability to directly recognize double-stranded DNA (dsDNA) via the formation of a unique invasion complex. While most natural and artificial nucleic acids form duplexes in an antiparallel configuration due to the general instability of parallel configurations, PNA distinctively forms both antiparallel and parallel duplexes. In this study, we focused on this previously underexplored property of PNA to adopt a parallel duplex configuration and developed a novel double-duplex invasion strategy by leveraging the differences in thermal stability between the antiparallel and parallel orientations of PNA duplexes. Furthermore,

SUBMITTER: Shibata M 

PROVIDER: S-EPMC12400189 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature