Scalable Biofabrication of Functional 3D Scaffolds via Synergy of Autopilot Single-Jet Electrospun 3D PCL Fiber Scaffolds and Cell-Laden Hydrogels.

ABSTRACT: 3D bioprinting enables cell-laden hydrogel construct fabrication in a layer-by-layer fashion but faces scalability challenges due to the mechanical weakness of hydrogels. Matrix reinforcement compromises cellular activity, creating a scalability-functionality trade-off that remains unresolved as sophisticated strategies including sequential and embedded printing fail to effectively overcome these limitations. This study presents an alternative approach by integrating autopilot single-jet electrospun (AJ-3D ES) 3D PCL fiber scaffolds with hydrogels, achieving anatomical precision, mechanical robustness, and enhanced cell function. Hydrogel dip-coating of anatomically structured PCL scaffolds enabled organ-scale cellular constructs. By providing an ECM-mimicking porous fiber network, embedde

SUBMITTER: Navaneethan B 

PROVIDER: S-EPMC12400274 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature