METTL3/YTHDF3 m⁶A axis promotes ferroptosis in diabetic kidney disease by stabilizing TfR1.

ABSTRACT:

Objective

Diabetic kidney disease (DKD) is a common complication of diabetes. N6-Methyladenosine (m⁶A) modification is a widely studied epigenetic mechanism. Methyltransferase-like (METTL) 3 is a well-studied methyltransferase. This study aimed to investigate the role of METTL3 in DKD and the underlying mechanism.

Methods

Thirty-five DKD patients and 28 control volunteers were recruited. Animal and cell DKD models were established. QRT-PCR and Western blot were performed to analyze the expression of METTL3 and fibrosis-related indicators. Cell viability and proliferation were assessed via a cell counting kit-8 and colony formation assays. Ferrous iron (Fe²⁺), malonaldehyde (MDA), and glutathione (GSH) contents were measured by commercial kits. The inter

SUBMITTER: Zhang J 

PROVIDER: S-EPMC12400356 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature