Extracellular vesicles from adipose-derived mesenchymal stem cells prevent high glucose-induced retinal ganglion cell pyroptosis through a microRNA-26a-5p-dependent mechanism.

ABSTRACT:

Objective

Mesenchymal stromal/stem cells have neuroprotective effects that limit damage to the retina, which is predominantly mediated by the released extracellular vesicles (EVs). This study aims to investigate the protective effect of adipose-derived mesenchymal stem cell-derived EVs (ADSC-EVs) against pyroptosis of retinal ganglion cells (RGCs).

Methods

ADSC-EVs were isolated and then characterized. Mouse primary RGCs exposed to high glucose (HG) were applied for in vitro experiments. miR-26a-5p expression in RGCs after ADSC-EV treatment was determined by RT-qPCR. Target relation between miR-26a-5p and histone deacetylase 4 (HDAC4) was identified by luciferase reporter assay. miR-26a-5p blockad and HDAC4 ectopic expression experiments were conducted to clarify their funct

SUBMITTER: Tang L 

PROVIDER: S-EPMC12400376 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature