Solvent Redistribution Method To Determine Solubility and Aggregation: High Throughput, Accuracy, and Sustainability.

ABSTRACT: The pharmaceutical industry is responsible for 4.4% of global CO₂ emissions. Assays used in drug discovery and development are major contributors to waste, including solubility measurements. These are either accurate but slow and energy-intensive (HPLC, centrifugation, filtration), contributing significantly to global CO₂ emissions, or fast and economical but inaccurate (turbidity/nephelometry). Here, we exploit the sensitivity of high-throughput angle-resolved second harmonic scattering to detect nanoscale interfacial fluctuations in the solvent around the solute. Classical nucleation theory and nonlinear light scattering modeling show that, prior to aggregation, the solvent interfacial area changes drastically. This leads to changes in the standard deviation of the

SUBMITTER: Tarun OB 

PROVIDER: S-EPMC12400408 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature