A Method for in Situ Interfacial pH Detection.

ABSTRACT: Functionalized silica-based surfaces are widely used across industries, from semiconductors to pharmaceuticals. Aminosilanes are commonly employed as coupling agents during surface functionalization to anchor diverse functional molecules. However, the surface modifications perturb interfacial physicochemical properties, resulting in a significant shift in interfacial pH compared to the bulk solution. This shift complicates direct measurement and accurate monitoring of interfacial conditions. To overcome this challenge, we functionalized glass surfaces with aminosilane-coupled pH-sensitive fluorescent dyes and utilized confocal microscopy to measure their fluorescence response to changes in bulk pH. Complementing these experiments, we developed a theoretical model describing equilibrium sur

SUBMITTER: Catalan KN 

PROVIDER: S-EPMC12400433 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature