Tuning probe permeability <i>via</i> chalcogen and halogen atom substitution for monitoring alkaline phosphatase activity in mammalian cells.

ABSTRACT: Alkaline phosphatase (ALP) is a family of hydrolase enzymes that play crucial roles in a wide range of biological processes. Its primary function is to catalyze the hydrolysis of phosphate groups from various molecules, a process known as dephosphorylation. It regulates diverse cellular functions such as bone metabolism and liver function. Alteration of ALP can be a biomarker for pathological conditions. Hence, the development of fluorescent probes for ALP detection is essential for studying dynamic dephosphorylation processes in living organisms. Herein, we report a ratiometric fluorescent probe based on a 1,8-naphthalimide derivative functionalized with an ALP-reactive phosphate group and a chalcogen or halogen atom as recognition moieties to enhance the probe internalization in mammalia

SUBMITTER: Chauhan E 

PROVIDER: S-EPMC12400448 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature