Exogenous Jaagsiekte sheep retrovirus (JSRV) Inner Mongolia strain: whole-genome characterization and viral particle packaging.

ABSTRACT:

Introduction

Ovine pulmonary adenocarcinoma (OPA) is a contagious lung tumor caused by the exogenous Jaagsiekte sheep retrovirus (exJSRV). Analysing the genome of the pathogen is crucial for developing OPA prevention and control measures. Due to the absence of exogenous genomic JSRV-related information in Inner Mongolia, we aimed to establish a specific technique for exJSRV genomic amplification.

Methods

Target virions were purified using U3 hn-PCR (hemi-nested PCR) combined with density gradient centrifugation. Specific reverse transcription primers were designed using the low-identity region of the internal and external genome, combined with long fragment PCR and 3'RACE technology, and the full-length genome of exogenous JSRV from Inner Mongolia was successfully obtained.<

SUBMITTER: Zhang P 

PROVIDER: S-EPMC12400676 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature