<i>Xist</i> upstream deletion leads to dysregulation of <i>Xist</i> and autosomal gene expression.

ABSTRACT: Xist long noncoding RNA is the master regulator of the X-Chromosome inactivation (XCI) process. Xist is expressed from the inactive X and coats the inactive X to facilitate XCI. Cis-regulation of Xist expression remains poorly understood in the context of maintenance of XCI. Here, we have explored the role of the Xist upstream sequences (∼6 kb) lying between Tsix and Jpx in the regulation of Xist and XCI in mouse extra-embryonic endoderm stem cells (XEN), which represent the maintenance phase of imprinted XCI. Here, we show that the deletion of this Xist upstream sequence in the inactive X leads to the upregulation of Xist expression accompanied by the dispersal of the Xist cloud. Notably, we find the loss of enri

SUBMITTER: Majumdar S 

PROVIDER: S-EPMC12400955 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature