Enabling 3D electrical stimulation of adipose-derived decellularized extracellular matrix and reduced graphene oxide scaffolds <i>in vitro</i> using graphene electrodes.

ABSTRACT: Notwithstanding the demonstrated benefits of electrical stimulation in enhancing tissue functionality, existing state-of-the-art electrostimulation systems often depend on invasive electrodes or planar designs. This work exploits the versatility of graphene to fabricate biocompatible electrodes for the three-dimensional in vitro electrical stimulation of neural stem cells. A conductive green graphene-based ink was formulated and screen-printed as the bottom and top electrodes in a bottom-less standard culture well plate. Upon exposure to macrophages, although some oxidative stress was observed, this graphene-based ink did not elicit an increase in the pro-inflammatory cytokine IL-6. An analysis of the electrode impedance as a function of time and frequency was performed to optimize

SUBMITTER: Martins PA 

PROVIDER: S-EPMC12400995 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature