Analysis of microbial diversity and functions in sediments and overlying water of the Shiliu River.

ABSTRACT:

Background

With the acceleration of urbanization, urban rivers have become a significant component of the urban ecosystem, attracting considerable attention regarding their ecological status and biodiversity. This study focuses on the Shiliu River, aiming to analyze the microbial diversity and functions present in the overlying water and sediments of severely polluted areas.

Methods

This study investigated the Shiliu River. In August 2024, sediment and overlying water samples were collected from its severely polluted reaches. The NextSeq 2000 PE300 platform was employed for sequencing to detect bacterial and fungal taxa abundances. PICRUSt and FUNGuild predicted sample functional abundances using bacterial 16S rRNA and fungal internal transcribed spacer (ITS) gene sequences,

SUBMITTER: Li Y 

PROVIDER: S-EPMC12401026 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature