Isolation of Nucleated Red Blood Cells With Intact Genomic DNA From Cord Blood by Applying G&T-Seq.

ABSTRACT:

Purpose

Fetal cells in maternal blood are a pure source of fetal genomic DNA for noninvasive prenatal testing (NIPT), if successfully isolated. We assessed whether single-cell genome and transcriptome sequencing (G&T-seq) can be applied to efficiently isolate fetal nucleated red blood cells (fNRBCs) suitable for genetic testing.

Methods

Using umbilical cord blood as a model, we isolated 165 single NRBC candidates from four samples and 12 single lymphocytes as controls from one sample. G&T-seq was used to estimate the maturation stage of each NRBC candidate from the transcriptome data, and genomic integrity was assessed using shallow whole-genome sequencing (WGS) data.

Results

Multi-dimensional scaling (MDS) of the transcriptome data revealed that five NRBC candidates

SUBMITTER: Ito N 

PROVIDER: S-EPMC12401139 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature