Molecular Detection and Isolation of <i>Bartonella</i> Species in Bats and Their Ectoparasites Along the China-Myanmar Border.

ABSTRACT: Bartonella are parasitic pathogens that infect many mammals, including humans, and cause significant diseases. This study investigates the presence, genetic diversity, and tissue tropism of Bartonella in bats and their ectoparasites along the China-Myanmar border. Bats and ectoparasites were collected from Yingjiang, Ruili, and Gengma Counties. Nested PCR (nPCR) and quantitative real-time PCR (qPCR) were used to detect and quantify Bartonella in bat tissues. Bartonella was isolated using brain-heart infusion broth and tryptone soy agar medium containing 5% sheep blood (TSA containing 5% sheep blood), and DNA sequences were analyzed with Clustal W and MEGA X. In total, 601 bats from 11 species (four families and seven genera) and 32 ectoparasites (two orders, thr

SUBMITTER: He C 

PROVIDER: S-EPMC12401608 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature