Black Cumin Seed (<i>Nigella sativa</i>) Confers Anti-Adipogenic Effects in 3T3-L1 Cellular Model and Lipid-Lowering Properties in Human Subjects.

ABSTRACT: Nigella sativa (black cumin seed) has traditionally been valued for its medicinal properties. This study explored its potential in addressing obesity-related conditions by assessing its anti-adipogenic and lipid-lowering effects. Black cumin seed extract showed high phenolic (35.48 mg GAE/g DW) and flavonoid (39.51 mg QE/g DW) contents with excellent standard curve linearity (R ² > 0.99). FTIR confirmed thymoquinone-related functional groups, and GC-MS revealed 23 fatty acids, predominantly methyl eicosatrienoate (69.29%), methyl 11,14,17-eicosatrienoate (25.2%), and methyl linoleate (4.05%). These results indicate a rich phytochemical and fatty acid profile. In vitro, 3T3-L1 preadipocytes were treated with a methanolic black cumin seed extract (BSE). Oil red O sta

SUBMITTER: Ahmed S 

PROVIDER: S-EPMC12401715 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature