Rapid and simple detection of <i>Listeria monocytogenes</i> using real closed dumbbell-mediated isothermal amplification.

ABSTRACT:

Introduction

Listeria monocytogenes (L. monocytogenes) is a well-known widespread food-borne pathogen that poses a threat to public health. Suitable detection methods are needed to effectively control and prevent pathogenic L. monocytogenes infections.

Methods

This study aimed to develop a novel closed dumbbell-mediated isothermal amplification (CDA)-based assay to achieve rapid and simple detection of L. monocytogenes. The newly developed CDA technology is capable of amplifying DNA targets with high sensitivity and specificity. The conserved hly gene of L. monocytogenes was taken as a target for the establishment of the CDA method. All primers were selected and evaluated by real-time fluorescence monitoring, and endpoint visual judg

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12401984 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature