Rational design of a multi epitope vaccine against Salmonella typhi via subtractive proteomics, reverse vaccinology and molecular modeling.

ABSTRACT: Salmonella enterica subsp. enterica serotype Typhi (Salmonella typhi) is the cause of typhoid fever, a severe public health issue in impoverished countries with inadequate sanitation. Despite the availability of therapies, infection rates remain high, underscoring the critical need for an effective and long-lasting vaccine. In this study, we used an integrated in silico strategy to develop a multi-epitope vaccine for 122 S. Typhi strains. A core proteome study identified 2,637 conserved proteins, while subtractive proteomics discovered three non-homologous, virulent, antigenic, and non-allergenic proteins: major curlin subunit, outer membrane protein A, and a hypothetical protein. Four B-cell and ten T-cell epitopes (four HTL and six CTL) were predicted and chosen for vaccine development u

SUBMITTER: Albutti A 

PROVIDER: S-EPMC12402470 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature