Anti-Apoptotic Effects of Escin on Porphyromonas gingivalis-Derived Lipopolysaccharide-Induced Injury in SH-SY5Y Cells.

ABSTRACT:

Background

Porphyromonas gingivalis-derived lipopolysaccharide (Pg-LPS) may be associated with the pathogenesis of Parkinson's disease and other neurodegenerative disorders. Escin is a bioactive compound found in the horse chestnut tree (Aesculus hippocastanum). This research investigated the impact of escin on Pg-LPS-induced injury in SH-SY5Y cells.

Methods

Parkinson's disease model was established using SH-SY5Y neuronal cells. Cell viability was evaluated with MTT assays. Cell morphology was observed by fluorescence microscopy. Apoptosis was detected by JC-1, TUNEL, and Hoechst/propidium iodide staining, and flow cytometric analysis. The mRNA levels of BCL2 and BAX were determined by RT-PCR, and the protein levels of BCL2/BAX, cleaved Caspase 3/Caspase 3, and PARP were eva

SUBMITTER: Sun Y 

PROVIDER: S-EPMC12402593 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature