Exploring the role of normalization and feature selection in microbiome disease classification pipelines.

ABSTRACT:

Background

Disease classification using 16S rRNA microbiome data faces challenges of high dimensionality, compositionality, and sparsity, compounded by the inherent small sample sizes in many studies. Machine learning and feature selection techniques offer potential to identify robust biomarkers and improve classification performance; however, their comparative effectiveness across diverse methods and datasets has been insufficiently explored. This study evaluates multiple feature selection techniques alongside normalization strategies, focusing on their interplay with classifier performance.

Results

Our analyses revealed that centered log-ratio normalization improves the performance of logistic regression and support vector machine models and facilitates feature selection,

SUBMITTER: Garach Velez I 

PROVIDER: S-EPMC12402773 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature