Detection of pathogens in dogs with respiratory disease during winter 2023-2024 using multiplex qPCR/RT-qPCR assays and next-generation sequencing.

ABSTRACT: Canine Infectious Respiratory Disease Complex (CIRDC), caused by a diverse range of viral and bacterial pathogens, is the leading cause of respiratory illness in dogs. In the winter of 2023-2024, the United States experienced a noticeable increase in cases consistent with CIRDC. This study investigated the potential association of emerging pathogens with CIRDC cases. It involved the analysis of 50 clinical specimens collected from CIRDC-suspected dogs from six US states between December 2023 and February 2024. All clinical cases presented with respiratory illness characterized mainly by coughing (78%), nasal and ocular discharges (30%), and sneezing (22%). Specimens were tested for 12 known CIRDC-associated pathogens using a previously described panel of one-step TaqMan^® multipl

SUBMITTER: Thieulent CJ 

PROVIDER: S-EPMC12402941 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature