MicroRNA-27a-3p targets FoxO signalling to induce tumour-like phenotypes in bile duct cells.

ABSTRACT:

Background & aims

Cholangiocarcinoma (CCA) is a heterogeneous and lethal malignancy, the molecular origins of which remain poorly understood. MicroRNAs (miRs) target diverse signalling pathways, functioning as potent epigenetic regulators of transcriptional output. We aimed to characterise miRNome dysregulation in CCA, including its impact on transcriptome homeostasis and cell behaviour.

Methods

Small RNA sequencing was performed on 119 resected CCAs, 63 surrounding liver tissues, and 22 normal livers. High-throughput miR mimic screens were performed in three primary human cholangiocyte cultures. Integration of patient transcriptomes and miRseq together with miR screening data identified an oncogenic miR for characterization. MiR-mRNA interactions were investigated by a luci

SUBMITTER: Duwe L 

PROVIDER: S-EPMC12402942 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature