A FRET assay to monitor different structural states of human β-cardiac myosin including the interacting-heads motif.

ABSTRACT: In cardiac muscle, myosin molecules exist in multiple structural states as they transit through their ATPase cycle, including an off-cycle resting or OFF-state with their catalytic heads in a folded structure known as the interacting-heads motif (IHM). The blocked head configuration (BHC) of the IHM is unusual because its light chain binding region is held in an exaggerated prestroke angle stabilized by interactions with its own S2 tail. An additional partial OFF-state, where the second head of the IHM is not folded back onto the blocked head, has been proposed, which still has the blocked head interacting with S2. Many mutations in the human β-cardiac myosin gene that cause hypertrophic cardiomyopathy are thought to destabilize (decrease the population of) the OFF-states. The effects of p

SUBMITTER: Goluguri RR 

PROVIDER: S-EPMC12403093 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature