Multiplexed Single-Molecule Detection of Nucleic Acid Biomarkers with a Distance-Tuned Single FRET Pair.

ABSTRACT: Biomarkers have gained tremendous attention in recent years, as they offer reliable detection of diseases such as cancers and other health conditions. However, with the recent realization that one biomarker can be associated with more than one disease (cross-talk), there is a significant shift toward simultaneous monitoring of more than one biomarker to increase the accuracy of diagnosis. Despite a sizable effort made over the last several years, multiplexing using the common techniques including surface-enhanced Raman spectroscopy (SERS), microarrays, RT-qPCR, nanostring, fluorescence, and others requires target amplification, target labeling, or the use of additional probes/actuators, and hence, these requirements complicate the experiments and data analyses. Using single-molecule fluore

SUBMITTER: Sethi S 

PROVIDER: S-EPMC12403156 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature