Circadian disruption and ROS-NLRP3 signaling mediate sleep deprivation-enhanced silica nanoparticle toxicity in lacrimal glands.

ABSTRACT: Sleep deprivation (SD) and exposure to engineered nanomaterials such as silica nanoparticles (SiNPs) are emerging risk factors for ocular surface disorders, particularly dry eye disease. However, the molecular mechanisms underlying their combined impact on lacrimal gland function remain unclear. In this study, we investigated the synergistic effects of SD and SiNPs exposure on circadian regulation, oxidative stress, inflammation, and structural integrity of the extraorbital lacrimal glands (ELGs) in C57BL/6J mice. Behavioral and physiological monitoring revealed that SD + SiNPs disrupted circadian locomotor activity and body temperature rhythms. Phenotypic assessments showed reduced tear secretion and ELG atrophy. RNA sequencing identified extensive transcriptomic reprogramming, including

SUBMITTER: Zhang W 

PROVIDER: S-EPMC12403269 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature