Isolation and molecular characterization of bacteriophages isolated from lake water and their application in foods against Bacillus cereus.

ABSTRACT:

Objectives

Bacteriophages are viruses that specifically target and kill bacteria. Bacteriophages are considered safe for humans, making them suitable for use in food applications Therefore, this study was conducted to isolate, characterize, and apply bacteriophages from the environment to control food spoilage bacteria.

Results

Bacteriophages were isolated by using Bacillus cereus (B. cereus) as reference host. We found bacteriophage BC-VP titers (1.16 ± 0.18 × 10⁹ PFU/mL) and BC-AJ (1.72 ± 0.19 × 10⁸ PFU/mL). Both bacteriophages were polyvalent, targeting not only B. cereus, but also lysing enteropathogenic Escherichia coli (EPEC) and enterotoxigenic Escherichia coli (ETEC) with different efficiencies. Bacteriophage BC-VP and BC-AJ miMOI were determine

SUBMITTER: Rizkinata D 

PROVIDER: S-EPMC12403466 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature