Probing Intracellular Yeast Metabolism With Deuterium Magnetic Resonance Spectroscopy.

ABSTRACT: Metabolomics provides snapshots of states of metabolites under specific conditions, with nuclear magnetic resonance (NMR) being one of the few noninvasive techniques. However, when applied to intact cells (e.g., yeast or mammalian cells) or tissues, traditional ¹H NMR often suffers from overlapping signals from numerous metabolites and intracellular macromolecules such as proteins. To address this, we employed deuterium-labeled tracers that do not suffer from background interference and streamline targeted flux analysis. Deuterium magnetic resonance spectroscopy (DMRS) enables rapid, noninvasive measurement of metabolic flux without specialized equipment. In our study, we first measured T₁, T₂, and chemical shifts for 26 deuterium-labeled compounds in phosp

SUBMITTER: Anum F 

PROVIDER: S-EPMC12403501 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature