Serological differentiation between naturally acquired mpox and MVA-BN-vaccine induced antibody responses using ratios of MPXV and VACV antigen pairs in the MSD immunoassay.

ABSTRACT: This study's aim was to evaluate and benchmark the gold-standard methods neutralization test (NT) and immunofluorescence (IF), against a model linked to a multiplex immunoassay method for analysis of the highly cross-reactive serological responses against the monkeypox (MPXV)- and vaccinia (VACV) viruses. Serum samples from men who have sex with men were analyzed (22 with previous PCR-confirmed mpox episode, 53 MVA-BN-vaccinated, 61 unexposed to infection/vaccination). In-house NTs and IF established titers of mpox- and vaccinia-specific neutralizing and binding IgG antibodies, respectively. The commercial Orthopoxvirus Panel 1 multiplex immunoassay (Meso Scale Discovery [MSD]) was used to measure IgG against five homologous MPXV and VACV antigens. Antibody titers were studied in relation

SUBMITTER: Pettke A 

PROVIDER: S-EPMC12403559 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature