Multiple cross displacement amplification (MCDA) for rapid detection of toxigenic <i>Clostridioides difficile</i> as a potential point-of-care testing.

ABSTRACT: Toxigenic Clostridioides difficile is the cause of C. difficile infection, highlighting the critical need for rapid and easy-to-use detection. In this study, a multiple cross displacement amplification (MCDA) assay was developed to detect toxigenic C. difficile and evaluated against real-time PCR and VIDAS C. difficile toxin A & B assay (CDAB). The results showed that MCDA targeting the C. difficile tcdB gene had a limit of detection (LOD) of 12.5 fg (2.5 copies of genomic DNA) per reaction with no non-specific amplification from other pathogens. The LOD for MCDA was the same as real-time PCR but had significantly faster detection time, detecting 500 pg and 12.5 fg per reaction in 5.7 ± 0.5 and 21.35 ± 2.2 min, respectively. Among 201 stool samples, MCDA

SUBMITTER: Hu H 

PROVIDER: S-EPMC12403592 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature