3D TGF-β1-expressing mesenchymal stem cells enhance wound healing in a diabetic mouse model.

ABSTRACT:

Background

Human mesenchymal stem cells (MSCs) are a promising stem cell source; however, their therapeutic efficacy in chronic wound healing remains limited. This study evaluates the therapeutic potential of transforming growth factor (TGF)-β1-modified, three-dimensionally cultured MSCs (A/T-3D) for enhancing wound healing.

Methods

The TGF-β1 gene was inserted into a safe genomic locus in adipose-derived MSCs (ASCs) using transcription activator-like effector nucleases. Quantitative polymerase chain reaction (qPCR) analysis showed that A/T-3D upregulated key factors related to wound healing, including epidermal growth factor (EGF), TGF-β1, fibroblast growth factor (FGF), and vascular endothelial growth factor (VEGF)-A, compared to unmodified ASCs.

Results

In vitro sc

SUBMITTER: Chae DS 

PROVIDER: S-EPMC12403617 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature