Characterization of strictly lytic phages infecting <i>Oenococcus oeni</i> from Merlot wines and proposal of a new genus.

ABSTRACT: Oenococcus oeni is the predominant lactic acid bacteria species in wine, where it performs the malolactic fermentation, which helps to secure and preserve wine quality. Here, we describe the morphological, biological, and genomic characterization of siphophage Krappator X₂₇, a strictly lytic phage that was previously isolated from Merlot wines. Several aspects of the life cycle of the phage were investigated using the sensitive strain IOEBS277 under optimal growth conditions. X₂₇ has a large burst size (149 Plaque Forming unit [PFU] per infected cell) and targets industrially relevant strains of O. oeni used for production of wines. X₂₇ also shows lytic activity against its host in red and white grape juice media. The phage genome consists of

SUBMITTER: Barchi Y 

PROVIDER: S-EPMC12403621 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature