Highly sensitive and multiplex detection of nine potential bioterrorism viral agents in a single reaction by multiplex probe amplification (MPA) with melting curve analysis.

ABSTRACT: The rapid and accurate detection of highly pathogenic viruses is critical for public health, especially in the context of potential bioterrorism threats. This study presents a novel multiplex probe amplification (MPA) assay combined with melting curve analysis for the simultaneous detection of nine high-threat viral agents: Ebola virus, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, Chikungunya virus, Monkeypox virus, Eastern equine encephalitis virus, Tick-borne encephalitis virus, and Venezuelan equine encephalitis virus. The MPA-nine-viruses assay utilizes three fluorescence channels and unique melting temperatures to differentiate between targets, achieving high sensitivity with a limit of detection as low as 1.5-15 copies/μL. The MPA-nine-viruses was high

SUBMITTER: Li Y 

PROVIDER: S-EPMC12403643 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature