Novel guanidine derivatives as mechanistic scaffolds for anticancer agents: synthesis, characterization, DNA-binding, and computational studies.

ABSTRACT: Cancer continues to be a major global health challenge, necessitating the ongoing development of novel small-molecule therapeutics that can selectively target DNA and disrupt cancer cell proliferation. In this study we report the synthesis and characterization of novel guanidine derivatives (7a-j). Their DNA-binding potential was assessed through electronic absorption spectroscopy, revealing characteristic hypochromic shifts indicative of minor groove-binding interactions with salmon sperm DNA (SS-DNA). Among the series, 4-Me, 4-Br-substituted compound (7i) exhibited the highest binding constant (K _b = 3.49 × 10⁵ ± 0.04 M^-1 at 298 K), comparable to that of the reference groove binder, cabozantinib (K _b = 5.79 × 10⁵ M

SUBMITTER: Zaman H 

PROVIDER: S-EPMC12403662 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature