Direct comparison of clear DX Nanopore and Illumina sequencing of SARS-CoV-2.

ABSTRACT: There are limited data directly comparing SARS-CoV-2 sequencing methods using two major commercial approaches, Oxford Nanopore Technologies Clear DX (ONTDX) and Illumina. RNA was extracted from 1,513 SARS-CoV-2 RNA-positive respiratory samples, split into two aliquots, and sequenced using both ONTDX and Illumina sequencing methods. FASTQ sequences generated by the ONTDX and Illumina (550 or 2,000) platforms used to test these samples were analyzed using either the same assembly and alignment strategy or using the platform default methodologies. ONTDX and Illumina sequencing results were not significantly different in generating consensus genomes, though slightly better coverage was obtained with ONTDX compared to Illumina. The results were similar using the harmonized assembly and alignmen

SUBMITTER: Hottel W 

PROVIDER: S-EPMC12403847 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature