Performance of direct detection of <i>Mycobacterium tuberculosis</i> within <i>Mycobacterium tuberculosis</i> complex by routine MALDI-TOF for diagnosis using species-specific lipid fingerprint.

ABSTRACT: Managing tuberculosis cases requires species and drug susceptibility identification, which are limited by the time taken by testing procedures due to slow bacterial growth. Lipid-based matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is a promising tool for identifying pathogenic mycobacterial species. This study aims to define and use species-specific lipid profiles of members of the Mycobacterium tuberculosis complex (MTBC) obtained by MALDI-TOF MS, to directly discriminate M. tuberculosis from other members of the MTBC, such as Mycobacterium africanum, Mycobacterium bovis, and M. bovis bacillus Calmette-Guerin (BCG). Reference strains (M. tuberculosis H37Rv, M. africanum, M. bovis, and

SUBMITTER: Cheong B 

PROVIDER: S-EPMC12403859 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature