Ontogenetic stage and type of donor cells shape extracellular vesicles' therapeutic potential for osteoarthritis.

ABSTRACT:

Background

Osteoarthritis (OA) remains an intractable condition due to the limited regenerative capacity of adult cartilage. Extracellular vesicles (EVs) have emerged as promising therapeutics, yet the optimal donor cell source is still undetermined, as both donor cell type and age significantly influence EV therapeutic efficacy. This study evaluates the therapeutic potential of EVs derived from ovine fetal articular chondrocytes (fCCs) and ovine fetal umbilical cord blood mesenchymal stromal cells (fMSCs) compared to EVs from two immortalized human perinatal cell lines, Wharton's jelly (WJ-MSCs) and amnion MSCs (P-MSCs), on inflamed ovine adult chondrocytes and synoviocytes in vitro.

Methods

EVs were isolated from conditioned media using tangential flow filtration and chara

SUBMITTER: Tarasova K 

PROVIDER: S-EPMC12403863 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature